5th Central-Eastern European Congress on Cell-Free Nucleic Acids
21-22 June 2018
Life Science Building, University of Debrecen, Debrecen

Program

Submitted lectures

Invited speakers

Howard Cuckle
Comparing different commercial cfDNA tests for aneuploidy
Department of Obstetrics and Gynecology, Tel Aviv University, Tel Aviv, Israel; Department of Obstetrics and Gynecology, Columbia University Medical Center, New York, NY, USA; Reproductive Epidemiology, University of Leeds, Leeds, UK
 
Erik Sistermans:
Introducing NIPT analysis in the Netherlands part II, from TRIDENT-1 to TRIDENT-2
VU University Medical Center Amsterdam, the Netherlands
 
Howard Cuckel.:
Invasive and non-invasive prenatal detection of micro-deletions
Department of Obstetrics and Gynecology, Tel Aviv University, Tel Aviv, Israel; Department of Obstetrics and Gynecology, Columbia University Medical Center, New York, NY, USA; Reproductive Epidemiology, University of Leeds, Leeds, UK
 
Ripudaman Singh1, Lotte Hatt1, Inga Baasch Christesen1, Katarina Ravn1, Olav Bjørn Petersen3,5, Niels Uldbjerg3, Rikke Christensen4, Ida Vogel4,5, Palle Schelde1
Fetal Cells in Maternal Blood for Prenatal Diagnosis – The story so far
1ARCEDI Biotech ApS, Vejle, Denmark.2 Aarhus University, Aarhus, Denmark. 3 Department of Obstetrics and Gynecology, Aarhus University Hospital, Aarhus, Denmark., 4 Department of Clinical Genetics, Aarhus University Hospital, Aarhus, Denmark. 5 Centre for Prenatal Diagnostics, Aarhus University Hospital, Aarhus, Denmark.
 
Folkert Asselbergs:
Cardiomyopathies: a genetic and phenotypic heterogeneous group of diseases
Department of Cardiology, Division Heart & Lungs University Medical Center Utrecht, the Netherlands
 
Christoph Sensen:
Circulating nucleic acids as early markers for infectious and chronic diseases
Institute of Computational Biotechnology,
Graz University of Technology, Graz, Austria
 
 
NIPT

Marian Grendár1, Dušan Loderer2,3, Iveta Švecová2, Zuzana Laučeková2, Michaela Hrtánková2, Andrea Hornáková3, Bálint Nagy4, Zora Lasabová5, Ján Danko2:
Decreasing the no call rate of  Non-Invasive Prenatal Testing
1. Bioinformatic unit, Biomedical Center Martin, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia, 2.Department of Obstetrics and Gynecology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia,3. Division of Molecular Biology, Biomedical Center Martin, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia,4. Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary,5. Division of Oncology, Biomedical Center Martin, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia
 
Bálint Tobiás1, Lili Készné Fodor1, Sarolta Szőke1, Gábor Molnár1, Valéria Klujber
Bernadett Balla1, Kövesdi Andrea1, Péter Lakatos1,2, János P. Kósa1,2:
PANORAMA® the SNP sequencing-based NIPT - Hungarian results in the light of American data
1PentaCore Laboratory, Budapest, Hungary,2 Semmelweis University, I. Dept. of Internal Medicine Budapest, Hungary
 
Darija Strah1, Nina Ružić Gorenjec2:
First trimester screening for Down’s syndrome by combined nuchal translucency and prenatal cf-DNA testing
 1. Strah Diagnostic Centre, Domzale, Slovenia, 2. Institute for Biostatistics and Medical Informatics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
 
Olga Török:
Cff-DNA based NIPTs in Hungary
Institute of Obstetrics and Gynecology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
 
Ilona Hromadnikova1,2, Katerina Kotlabova1,2, Katarina Ivankova2, Ladislav Krofta2:
First trimester screening of circulating C19MC microRNAs and the evaluation of their potential to predict the onset of preeclampsia and FGR
1 Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague, Czech Republic,2 Institute for the Care of the Mother and Child, Third Faculty of Medicine, Charles University, Podolskenabrezi 157/36, 147 00 Prague, Czech Republic
 
Ilona Hromadnikova1,2, Katerina Kotlabova1,2, Ladislav Krofta2, Filip Hron 2:
Follow-up of gestational trophoblastic disease/neoplasia via quantification of circulating nucleic acids of placental origin using C19MC microRNAs, hypermethylated RASSF1A and SRY sequences
1 Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University,Ruska 87, 100 00 Prague, Czech Republic,2 Institute for the Care of the Mother and Child, Third Faculty of Medicine, Charles University,Podolskenabrezi 157/36, 147 00 Prague, Czech Republic
 
 
Non-invasive detection of heart- and cardiovascular diseases

Zoltán Csanádi:
Electrical storm in the brain and in the heart: Epilepsy and Brugada syndrome
Institute of Cardiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
 
Orsolya Biró, Bálint Nagy:
Non-invasive prenatal testing for congenital heart disease – cell-free nucleic acid and protein biomarkers in maternal blood
1First Department of Obstetrics and Gynaecology, Semmelweis University, Budapest, Hungary,2Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
 
Orsolya Biró1, János Rigó Jr.1, Bálint Nagy2:
Maternal methylenetetrahydrofolate reductase (MTHFR) polymorphisms: risk factors for congenital heart disease?
 1First Department of Obstetrics and Gynaecology, Semmelweis University, Budapest, Hungary
2Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
 
 
Non-invasive detection of cancer

Péter Igaz:
Circulating microRNAs and tumors
2nd Department of Medicine, Semmelweis University, Budapest, Hungary
 
Gergő Papp1; Ilona Kovalszky1; Csaba Bödör1,2:
The role of circulating cell-free DNA as a cancer biomarker
1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary,MTA-SE Lendület Molecular Oncohematology Research Group, Budapest, Hungary,1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
 
Róbert Póka:
Free nucleic acids in ovarian cancer
Institute of Obstetrics and Gynecology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
 
Pál Perge1, Henriett Butz2, Raffaele Pezzani3, Irina Bancos4, Zoltán Nagy1, Ábel Decmann1, Michaela Luconi5, Massimo Mannelli5, Edit I. Buzás6, Miklós Tóth1, Marco Boscaro3, Attila Patócs2,7, Peter Igaz1,6:
Evaluation of circulating exosomal microRNAs in benign and malignant adrenocortical tumors
12nd Department of Medicine, Semmelweis University, 1088 Budapest, Szentkirályi str. 46, Hungary,2Molecular Medicine Research Group, Hungarian Academy of Sciences and Semmelweis University, 1088 Budapest, Szentkirályi str. 46, Hungary,3Endocrinology Unit, Department of Medicine, University of Padua, Via Ospedale, 105, 35128 Padova, Italy,4Division of Endocrinology, Diabetes, Metabolism and Nutrition, Department of Internal Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905 USA, 5Department of Experimental and Clinical Biomedical Sciences, Endocrinology Unit, University of Florence, VialePieraccini 6, 50139 Florence, Italy, 6Department of Genetics, Cell- and Immunobiology, Semmelweis University, 1089 Budapest, Nagyvárad tér4., Hungary, 7“Lendület-2013” Research Group, Hungarian Academy of Sciences and Semmelweis University, 1088 Budapest, Szentkirályi str. 46, Hungary
 
Gábor Méhes, Judit Bedekovics, Attila Mokánszki:
Analysis of oncogenic mutations from liquid biopdy samples: potentials and limitations in oncology
Department of Pathology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
 
Ilona Kovalszky, Lászlóné Oláh, Krisztina Császár, Krisztina Egedi, Csaba Bödör:
Our first experiences with testing EGFR T790M resistance mutation in liquid biopsy
Semmelweis University 1st Department of Pathology & Experimental Cancer Research, Budapest, Hungary
 
Vilmos Adleff1, Jillian Phallen1, Alessandro Leal1, Brian Woodward2, Patrick M. Forde1, Kristen A. Marrone1, Julie R. Brahmer1, Jacob Fiksel1, Stephen Cristiano1, Daniel C. Bruhm1, Elizabeth Weihe2, Valsamo Anagnostou1, Robert B. Scharpf1, Hatim Husain2, Victor E. Velculescu1:
Cell-free tumor load as possible predictive marker for TKI response in NSCLC therapy
1 The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA, 2 Division of Hematology and Oncology, Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093, USA
 
Zora Lasabová1*, Tatiana Burjanivová2, Bibiana Malicherová1,3, Marián Grendár4, Barbora Váňová, 1,2,Lukáš Plank1,3:
Non-invasive detection of biomarkers in plasma of cancer patients using droplet digital PCR – overview, first experiences and perspectives
1Division Oncology, Biomedical Center Martin, Comenius University in Bratislava, Slovakia,Jessenius Faculty of Medicine in Martin, Slovakia,2Department of Molecular Biology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Slovakia,3Department of Pathological Anatomy, Comenius University in Bratislava, Slovakia,Jessenius Faculty of Medicine and University Hospital in Martin, Slovakia,4Department of Bioinformatics, Biomedical Center Martin, Comenius University in Bratislava, Slovakia,Jessenius Faculty of Medicine in Martin, Slovakia
 
Judit Keserű1, Beáta  Soltész1, János Lukács2, Róbert Póka2, Bálint Nagy1:
Correlation of plasma mitochondrialDNA copy number and status of ovarian cancer patients
1Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary,2Institute of Obstetrics and Gynaecology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
 
Melinda Szilágyi-Bónizs1, Éva Márton1, János Lukács2, Beáta Soltész1, Eszter Janka3, András Penyige1, Róbert Póka2, Bálint Nagy1:
MiR200a, miR200b and miR200c are promising candidate biomarkers in epithelial ovarian cancer
1 Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary,2Department of Obstetrics and Gynecology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary,3Department of Dermatology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
 
Beáta Soltész1, János Lukács2, Edina Szilágyi1, Róbert Póka2, Bálint Nagy1:
Cell-free, long non-coding RNA in the diagnosis of ovarian cancer
1Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary, 2Institute of Obstetrics and Gynecology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
 
Naci Cine1, Nilufer Sertdemir1, Duygu Yavuz1, Merve Gokbayrak1, Eda Guzdolu1, Gulhan Demir1, Devrim Cabuk2, Ulas Isik2, Ercan Ozden2, Kazim Uygun2,Gorkem Aksu3, Hakan Savli1:
NGS based ctDNA-liquide biopsy analysis in Turkish cancer patients
1. Kocaeli University Faculty of Medicine, Department of Medical Genetic, Izmit, Turkey,2. Kocaeli University Faculty of Medicine, Department of Medical Oncology, Izmit, Turkey,3Kocaeli University Faculty of Medicine, Department of Radiation Oncology, Izmit, Turkey
 
Ozkan Ozden, Ilhami Gok:
Deacetylation of JAK1 and Its Association with IL-6-Induced Inflammatory Response in Cancer
Kafkas University, Faculty of Engineering and Architecture, Department of Bioengineering Central Campus, 36100, Kars, TURKEY
 
Beáta Soltész1, Orsolya Biró2, János Lukács3, András Penyige1, Róbert Póka3, Bálint Nagy1:  Cell-free miRNAs as potential biomarkers in the diagnosis of ovarian cancer
1Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary,  2Department of Obstetrics and Gynecology,Semmelweis University, Budapest, Hungary, 3Institute of Obstetrics and Gynecology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
 
Detection of infectious diseases and others topics
Tomáš Szemes1,3,5, Andrej Baláž 2,3,5, Michal Kajsík1,2, Iveta Gazdaricová2, Orsolya Bíró4, Bálint Nagy4, Ján Turňa1,2, Jaroslav Budiš2,3,5:
Are phages part of our immunity?
1Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia, 2University Science Park; Comenius University, Bratislava, Slovakia, 3Faculty of Mathematics, Physics and Informatics, Comenius University, Bratislava, Slovakia,4 Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary, 5Geneton Ltd; Bratislava, Slovakia
 
Aleksandar Vojta, Goran Josipović, Vanja Tadić, Vlatka Zoldoš::
Editing the epigenome using a comprehensive CRISPR/Cas9-based molecular toolbox
Department of Biology, Faculty of Science,University of Zagreb, Zagreb, Croatia
 
Peter Celec, Barbora Vlková:
Causes and consequences of the variability of extracellular DNA
Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia
 
Emese Domonkos, Ľubica Janovičová, Manuel Steinhardt, Tim Höpfner, Thomas Stehle, Peter Celec:
Sex differences in plasma deoxyribonuclease activity in rats
Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Sasinkova 4, Bratislava, Slovakia
 
Ľubica Janovičová, Barbora Konečná, Jozef Čonka, Lucia Lauková, Peter Celec:
Variability in deoxyribonuclease activity and extracellular DNA in mice
Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia
  
Gergely Buglyó1, Zsófia Magyar2, Éva Romicsné Görbe2, Rita Bánusz3, Monika Csóka3, Tamás Micsik4, Zsanett Berki1, Péter Varga2, Zoltán Sápi4, Bálint Nagy1:
Novel miRNA downregulations in blastemal Wilms’ tumor: an FFPE-based study
1Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary,21st Department of Obstetrics and Gynaecology, Semmelweis University, Budapest, Hungary,32nd Department of Paediatrics, Semmelweis University, Budapest, Hungary,41st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary
 
Posters

Hrindová B., Matějčková I., Zembol F., Bittóová M., Koudová M., Stejskal D.:
Automation of Cell-Free DNA Testing on Ion Proton System
Centre of Medical Genetics and Reproductive Medicine, Gennet, Ltd., Kostelní 9, Prague 7, Czech Republic
 
Éva Márton1, János Lukács2, Réka Szabó1, Beáta Soltész1, Eszter Janka3, Róbert Póka2, Bálint Nagy1, Melinda Szilágyi-Bónizs1
Study the role of miR141 and miR429 in the diagnosis of epithelial ovarian cancer
 1 Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary, 2Department of Obstetrics and Gynecology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary, 3Department of Dermatology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary
 
Sayli A, Akdeniz E, Araz I, Kahveci N, Kokturk G, Ture N, Bektas M,  Serim G, Bilgin Z, Ozdemir D.:
Determination of Common Chromosomal Abnormalities with NIFTY Test  (Non-invasive Fetal Trisomy Test) in Turkey
GENOKS Genetic Diagnostic Center, Ankara, Turkey
 
Krisztina Szirák 1, Beáta Soltész1, Orsolya Hajas2, Réka Urbancsek2, Edina Nagy-Baló2, András Penyige1, Zoltán Csanádi2, Bálint  Nagy1:
Determination of PITX2 and NEURL1 single nucleotide polymorphisms in atrial fibrillation
1University of Debrecen, Faculty of Medicine, Department of Human Genetics, Debrecen, Hungary; 2University of Debrecen, Faculty of Medicine, Institute of Cardiology, Debrecen, Hungary
 
Katarina Zelinova1,2, Marianna Jagelkova1,2, Zuzana Laucekova2, Zuzana Dankova1, Marian Grendar3, Karol Dokus2,4
Comparative analysis of the somatic mutation profiles in monitored 26 genes in circulating tumor DNA from breast cancer patients before, after surgery and after adjuvant chemotherapy – the pilot study
1 Department of Oncology, Biomedical Center Martin, Jessenius faculty of medicine in Martin, Comenius University in Bratislava (JFM CU), 036 01Martin, Slovakia, 2  Department of Obstetrics and Gynecology, Martin University Hospital and JFM CU, 036 01 Martin, Slovakia, 3 Department of Bioinformatics, Biomedical Center Martin JFM CU, 036 01 Martin, Slovakia, 4  II. Department of Obstertrics and Gynecology, Slovak Medical University, Faculty Hospital with Polyclinic of  F. D. Roosevelt, 975 17 Banska Bystrica, Slovakia
 

Company presentations:

Zhou Yafeng:
Introduction NIFTY test (NIPT) and comparison of the q-PCR technology with NGS technology
BGI International Dx Unit
 
Zhou Yafeng:
Introduction our Sentis(cancer panel) with our own sequencer of NGS technology
BGI International Dx Unit